This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
We define chemical reaction is a process in which old bonds break and new bonds are formed . So from the definition you can get the result easily
Answer:
Ture
For work, in Science to be done a force must be exerted and there must be motion or displacement in the direction of the force
Answer:
the answer is c , contains the hereditart blueprint of the cell
