Idk honestly that’s some bs I don’t even know what physical means
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Answer:
When the pKa is 6.0, we can determine the fraction of protonated H is by:
pH = pKa + log [A]/[HA]
Where
A = Deprotonated imidazole side
HA = Protonated side
Given, pH = 5.0
5 = 6 + log [A]/[HA]
log [A]/[HA] = -1 (take antilog of both side)
[A]/[HA] = 0.1
The ratio of the deprotonated imidazole side chain to the protonated side chain at pH 5.0 = 0.1
Given, pH = 7.5
7.5 = 6 + log [A]/[HA]
log [A]/[HA] = 1.5 (take antilog of both sides)
[A]/[HA] = 31.62
The ratio of the deprotonated imidazole side chain to the protonated side chain at pH 5.0 = 31.62
