When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
The rose family is a medium-sized family of flowering plants. The rose family (Rosaceae) is one of the major angiosperm families. The leaves are generally arranged spirally. Flowers of plants in the rose family are generally described as "showy". They are actinomorphic and almost always hermaphroditic. Rosaceae generally have five sepals, and five petals. They also have many spirally arranged stamens. The bases of the sepals, petals, and stamens are fused together to form a cup-like structure called a hypanthium. They can be arranged in racemes, spikes, or heads; solitary flowers are rare.
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They are known as the power house of the cell. They are organeles that act as a digestive system which then they take in nutrients, break them down, and create energy for the cell. This process is also known as cellular respiration.
