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Readme [11.4K]
3 years ago
15

What is the expected colorimetric result from the negative control in your assay?

Biology
1 answer:
mote1985 [20]3 years ago
5 0
<span>3,3',5,5'-Tetramethylbenzidine or TMB is a chromogenic substrate visualising reagent used in enzyme-linked immunosorbent assays (ELISA), it yields a blue color when oxidized, typically as a result of oxygen radicals produced by the hydrolysis of hydrogen peroxide by HRP, (thermofisher). The expected colorimetric result from the negative control in your assay will be no development of blue color</span>
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Adaptive defenses include humoral immunity only. Memory B cells areproduced as soon as the B cell binds to an antigen. Adaptive
Rina8888 [55]

Answer:

Adaptive defenses include both humoral and cellular immunity.

Explanation:

The innate immune response is the non-specific immune response and serves to provide an immediate and general immune response. The adaptive immune responses are the specific immune responses. Adaptive immune responses include cell-mediated immunity and antibody-mediated immunity.

Cell-mediated immunity includes T cells (cytotoxic and helper T cells) while antibody-mediated immunity includes the production of antibodies from B cells. Binding of antigen to B cells is followed by their transformation into plasma cells and produce antibodies. Some of the activated B cells form memory B cells that are responsible for quicker and strong secondary immune responses.

5 0
3 years ago
In an analysis of the nucleotide composition of DNA, which of the following proportions will be found?
katrin [286]

Answer:

With respect to the composition of DNA, in an analysis it can be found that the proportions of nucleotides are A + C = G + T (option a).

Explanation:

The proportion of nucleotides in a DNA molecule can be established according to the sequence of these nucleotides on both complementary strands, since the purinic bases of one strand are complemented by the pyrimidinic bases of another:

  • <em>Adenine is complemented with Thymine A=T</em>
  • <em>Guanine is complemented with Cytosine G=C</em>

From this we can deduce that in a DNA molecule with two chains there will be the same amount of adenine and thymine, as well as the same number of guanine with respect to cytosine, so:

<em>     A + C = G + T</em>

An example of this would be a known DNA molecule (hypothetical), with 5 molecules of Adenine and 7 molecules of Guanine. In this case there are 5 Thymines, complementary with Adenine, and 7 Cytosines like Guanine:

<em>     A + C = G + T</em>

<em>     5 + 7 = 7 + 5</em>

6 0
3 years ago
A food worker needs to thaw a package of ground beef
Hunter-Best [27]

Answer:

Under cold water

Explanation:

This is how me and my family thaw beef

8 0
2 years ago
Choose all the answers that apply. Metamorphic rock can form from __
andrew11 [14]
I think its c. igneous rock

5 0
3 years ago
Read 2 more answers
Which is the reproductive method of prokaryotes?
Verizon [17]

Answer:

Prokaryotic cells reproduce by a process that is called binary fission.

Explanation:

The DNA in such cells is contained in a single circular chromosome called a plasmid within the cytoplasm. The reproductive process starts with the replication of the chromosome.

5 0
3 years ago
Read 2 more answers
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