Answer:
The correct answer is - during active cellular growth and after cell lysis
Explanation:
Endotoxins, also known as LPS, are part of the outer leaflet of the outer membrane of the cell wall of gram-negative bacteria. These LPS especially Lipid A LPA released into the circulation when there is a disruption of the intact bacteria or after cell lysis. These are present in cell-associated bacteria such as Gram-negative pathogens such as Escherichia coli, Salmonella, Shigella, Pseudomonas, and others.
Answer:
The first anticodon leaves the ribosome through the E-site
Explanation:
Translation is the second stage of gene expression. It occurs in the ribosomes (organnelles of protein synthesis) where amino acid sequence is synthesized using a mRNA template. The tRNA (transfer RNA) is responsible for reading the mRNA codon using its ANTICODON, which is complementary to the mRNA codon.
The tRNA reads the mRNA codon and carries the amino acid that corresponds what it reads. tRNA has three binding sites on the ribosome; A-site, P-site and E-site. A tRNA molecule with the complementary anticodon binds to the codon on the P-site, and carries its corresponding amino acid. Another tRNA with complementary anticodon occupies the A-site, carrying the corresponding amino acid again.
Once both sites are occupied, the tRNA on the P-site transfers its amino acid to the one on the A-site to form a peptide bond. This causes the ribosome to shift the tRNA on the P-site, allowing the one on the A-site to be free. When this happens, the anticodon of the first tRNA on the P-site leaves the ribosome via the E-site in order to bind to another complementary mRNA codon and continue the translation process.
