Answer:
The correct answer is -
II: lyse cells of interest, precipitate DNA
V. Digest (cut) genomic DNA with restriction enzyme
I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!
IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.
III Introduce constructs via transformation into competent E. coli cells.
Explanation:
The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.
The genomic library construction involved some major steps that are:
- Extract and purify DNA - lysing and separating the desired DNA of interest.
- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes
- cut phage (chromosome) with restriction enzyme
- Insert the fragments of DNA into vectors
- introduce these recombinant a host bacterium by transformation, creating a DNA library.
Answer:
use own words!!!
Explanation: Velocity increases as more water is added to rivers via tributary rivers. This means that less of the water is in contact with the bed of the river and the mouth so there is less energy used to overcome friction. Hence rivers flow progressively faster on their journey downstream.
True. They have tools to do so.
I hope i helped!
