When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
The particles that cause the aurora are energized in the magnetosphere, that is, above the atmosphere. If they don't reach 10,000 volts, they will still cause auroral light. The lower energy won't change the wave length of the light, but will cause the light to be dimmer.
Explanation:
Answer:
plasma membrane/ cell membrane
