Answer:
C ( sucrose converts to glucose and fructose )
Explanation:
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When every dilution of your sample results in a plate that is too few to count we should filter the original sample and use most probable number method .
Probable number method count and determine the number of bacteria in a culture, plate aliquots of the dilutions onto agar with sterile pipettes, spread with glass sticks, incubate at 37°C and count the number of colonies. It is imperative that you utilize your best culture technique.
Not all bacterial cells produce colonies, as some bacteria tend to clump or aggregate, and some are nonviable. For this reason results are reported as colony forming units (CFU)/ml of bacterial culture. Ideally only plates with 25-250 colonies are used.
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In step 2: Denaturation of the double-strand occurs.
In step 3: Annealing of the primer to the single strands.
In step 4: Extension or elongation takes place in this step.
Explanation:
In the PCR program an enzyme Taq Polymerase is used because it can withstand high temperature without altering its functions.
PCR is required for the amplification of DNA into multiple copies for experimental purpose. The artificial environment is created to form new DNA molecules from the sample in question.
The first step in replication is the opening of the double helix which is done by temperature treatment in PCR. The temperature would be 90 degrees for some 30 sec to two minutes.
The next step of primer annealing would be done at 52 degrees, this is the primer melting temperature.
The elongation of the DNA strand to be synthesized will take place at 72 degrees as Taq Polymerase can withstand that temperature.
Nearly one million copies of DNA will be made after 30 cycles of PCR.
PCR products can be stored at 4 degrees for some two months.
Its c because all the genes add up and she gets wavy hair