Manifest destiny: A widely held belief in the 19th century United States that its settlers were given the divine right to expand across the continent.
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One reason conquistadors failed to establish colonies is that the Natives kicked them out. Many of the aboriginal people were very powerful and could easily get rid of the conquistadors.
Conquistadors could also run out of resources. Nothing lasts forever, and if they couldn't obtain enough resources while exploring and settling it would be hard to establish a colony easily.
There could also be dangerous things that could hinder the settlements such as wild beasts, natural events, and diseases.
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I'd say its the first one.
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