The answer to this would be none of the above. The temperature of the office is what will most likely be a problem for Ashley. This type of barrier is more of an environmental factor. Other environmental factors that can affect effective listening are lighting, furniture and the like. They become a barrier because they become distractions. People tend to get distracted when they grow uncomfortable, in this case it is physical discomfort.
To make it easier for people to answer your question, you should separate the questions from the answers, because I can't understand where the choices are and what the questions are.