When a drug is no longer effective but an organism is susceptible in vitro, it is called intermediate.
<h3>What is it known as when a drug loses its effectiveness but an organism is still susceptible in vitro?</h3>
- When a bacterial strain is susceptible in vitro to a concentration of an antibiotic drug that is linked to a questionable therapeutic effect, it is said that the bacterium's sensitivity to that antibiotic is intermediate. Thus, When a drug is no longer effective but an organism is susceptible in vitro, it is called intermediate.
- The designation "intermediate" suggests that while the same antibiotic may not be sufficiently effective against the same organism if it is located in other places, such as the meninges, it may readily be eliminated in bodily compartments that are easily accessible by the medicine, such as the urinary tract.
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Answer:
Petri plates are incubated upside down to prevent contamination.
Explanation:
The plates are always incubated inverted or turned upside down on their covers during storage. This is done to prevent evaporation if plaque is stored for long periods, which can affect the growth efficiency of bacteria, or allow contamination by multiplying unwanted organisms such as mold.
Once the plaques have been filled with a damp suspension of bacteria, it should be allowed to evaporate shortly before overnight incubation. However, a moderate amount of moisture will still be present. If plaque is not inverted during incubation, bacteria will not be able to attach to the culture medium properly, which will either prevent them from growing and forming colonies properly or will encourage the growth of undesirable microorganisms.
Any of these results will invalidate the experiment. In addition, any condensation or moisture can cause streaking, which will make it difficult to select and analyze separate colonies. After bacteria form colonies, plaque is also stored upside down to maintain moisture levels.
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