During the Persian Wars, the Greek army was weakened and then defeated by the Persians. This led to a decrease in the influence of Greece in Asia Minor.
During this time, Macedonia was one of the strongest states in Greece, and it had control over most of mainland Greece. The empire that emerged from this period is called Macedonian Empire. This empire lasted for another 300 years until its conquest by Rome around 148 BC.
The rise of Macedonia as a major power during this period also gave way to many internal conflicts between different tribes within the kingdom which led to increased military spending on them (cavalry). However, when Roman legions were sent into Asia Minor they easily crushed these armies and destroyed much of their infrastructure including fortifications and roads (roads were built under Alexander's reign but they fell apart quickly). This made it very difficult for any future armies or empires to hold onto territory once conquered.
So I think your question has been answered! Thanks!
