Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Decreases... barely any energy is transferred to the next level and most of it is lost through heat
1. A logging company plants two acres of land with
trees for every one acre of forest it removes -B. using resources sustainably-
2. Julia uses old glass jam jars as pots for
her plants-C. recycling resources-
3. Jackson installs a solar-powered water heater
to supply his house with hot water-A. using renewable resources-
<span>Metamorphic rocks form by existing rocks join together under high heat and pressure. Examples of metamorphic rocks are marble, schist, slate, and quartzite. Metamorphic rocks formed when the minerals are chemically changed due to heat and pressure. They are often seen near magma but they do not melt like igneous rock.</span>
No lesions in the mouth and throat
Weight increase of 1 lb over 3 days Correct
Temperature change from 100.2° F to 99.2° F
Capillary refill slowing from 2 seconds to 3 seconds
Weight increase of 1 lb (0.45 kg) over 3 days
Rationale: Vomiting results in fluid volume deficit. The most accurate method of evaluating fluid volume increase (the desired outcome) is weight. A temperature decrease is not reflective of fluid volume increase. Increasing capillary refill time is indicative of a fluid volume decrease, not an increase. The absence of mouth ulcers would allow the child to drink without pain but does not reflect a fluid volume increase.
