Answer:
Explanation:
Liquid-liquid extraction is a very useful method to separate components from a mixture. It consists of separating one or several substances dissolved in a solvent by transferring them to another solvent insoluble or partially insoluble in the first. The transfer of matter is achieved by direct contact between the two liquid phases.
For the extraction process, the solution is placed in a separating funnel, a water-immiscible organic solvent is added (ethyl ether is the most used), the solution with the compound to be separated, the funnel is covered and the funnel is top. Then it shakes. Depending on the solubilities and density, different layers are observed. The denser the compound, the more it will sink.
Since the organic compound is usually much more soluble in ether than in water, most of the organic compound will be dissolved in the ether phase (upper phase) and inorganic salts, which are not soluble in ether, will remain in the aqueous phase ( lower phase). Subsequently, by separating the separating funnel the two phases are separated, the organic phase is collected.
Occasionally, after stirring, the two immiscible liquids do not separate sharply, forming an emulsion in the intermediate zone. This is called the colloidal suspension of a liquid in another (system consisting of two or more phases, usually a liquid and another dispersed in the form of generally very fine solid particles). One of the reasons for the formation of an emulsion is when the two phases have similar densities. Then the relative density of the organic solvent and water cannot always be relied upon, although there are methods to facilitate the complete separation of the two phases.
POH + pH = pKw
pOH + pH - pH = pKw - pH
pOH = pKw - pH.
The correct equation used to calculate pOH would be C. pOH = pKw - pH.
Answer:
High concentration of glutathione should be included in the elution buffer for the given experiment to remove protein from the column.
Explanation:
Affinity chromatography is one of important biochemical technique of chromatography which depends on the affinity of ligand for the receptor.Here the ligand is a protein mixture which act as mobile phase and the receptor is present in the wall of chromatography column act as stationary phase.When the protein mixture is applied on the top of the column the substances present within the protein that have high affinity for the receptor present in the walls of chromatography column binds to the later but rest of the protein pass away through the column.High concentration of ligand is used within an buffer solution to remove the desired protein from the column.
From this point of view it can be stated that in the given question high concentration of glutathione should be used to remove the desired protein from the column.