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Nutka1998 [239]
2 years ago
5

PLS HELP! WILL GIVE BRAINLIEST!

Biology
1 answer:
Sonja [21]2 years ago
3 0

Answer:

10 percent

Explanation:

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The CRISPR/Cas9 system can cleave genomic DNA at sequences other than the desired target, a phenomenon referred to as off target
Deffense [45]

Answer:

The minimum length of a sgRNA sequence to avoid off target cleavage by the CRISPR/Cas system in the fly fruit genome is 14 bases

Explanation:

We are trying to use the CRISPR/Cas system to cleavage the genome of the fruit fly (which is 1.4x10^8 bp long). Also we desire the cleavage to be unique. That means we need a target sequence long enough to be able to assume it will only appear once in the genome.

First, we should think that in every position, we can find one out of four different nucleotide (A, C, T, G). So, the probability of getting a sequence of a given length "n" will be (1/4)^n (We are assuming that the probability of finding a nucleotide in the position "i", it's independent of the nucleotide we find in any other position "j").

Also, to know how many times a sequence will appear in a genome (the expected value of occurrence), we must multiply the probability of that sequence to randomly occur by the length of the genome. For our specific example, the number of occurence of a sequence of length "n" is:

nºoccurence=[(1/4)^n]*1.4*10^8

But in this case, what we want is the expected number of times the sequence will appear to be 1, and we want to obtain the length of the target sequence (n).

Given the information above, we know that:

[(1/4)^n]*1.4*10^8 =1

[(1/4)^n]=(1/1.4*10^8)=1.4*10^-8

Then, if we want to calculate n, we can use logarithms and its properties to get:

log[(1/4)^n]=log[1.4*10^-8]

n*log[(1/4)]=log[1.4*10^-8]

n=log[1.4*10^-8]/log[(1/4)] => n=13.29 approximately.

As the sequence needs to have a natural number of elements, <u>we can conclude that using a target sequence of a minimum of 14 bases with the CRISPR/Cas system in the fly fruit genome should be enough to avoid off target cleavage.</u>

3 0
3 years ago
What happens when the organic matter of the fossil is replaced by stone
wolverine [178]
Petrifaction happens jvgocg igcgicigcicg
3 0
2 years ago
Give an example of how an organism can use surface tension?
aliya0001 [1]
Insects, such as water striders, and spiders, such as fisher spiders, can glide or scamper across the surface of water. These critters are small enough that their weight can be supported almost entirely by surface tension, the relatively weak forces that hold water molecules together.




Surface tension of water helps creatures(mostly of insecta class such as water striders) to walk on water. ... it also helps water to move up the xylem tissue of higher plants without breaking up
3 0
2 years ago
Can someone help me <br> A. Enzyme<br> B. Starch<br> C. Oil<br> D. DNA
Sphinxa [80]

Answer:

The answer is A. Enzyme

Explanation:

7 0
3 years ago
Read 2 more answers
Schizogony is an important aspect of which of the following diseases?A. Chagas diseaseB. schistosomiasisC. toxoplasmosisD. plagu
Gwar [14]

Answer:

E. Malaria

Explanation:

Schizogony refers to the process of asexual reproduction through the process of multiple fission. The malaria parasite, <em>Plasmodium</em> species, enters the human body through the bite of female anopheles mosquitoes.

These mosquitoes carry haploid sporozoites in their saliva which are released into the bloodstream of human. The haploid sporozoites enter the liver cells where they multiply and increase their number through the process of multiple fission.

The process of schizogony in sporozoites of malaria parasite produces merozoites which are then released from the liver cells.

8 0
3 years ago
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