There are no positive connotation for glare would be look.
I would say the correct answer is - <span>But the doctor's four friends had taught no such lesson to themselves. They resolved forthwith to make a pilgrimage to Florida, and quaff at morning, noon, and night, from the Fountain of Youth.
As you already said, allegory is something that tells us a deeper and more important story than what may seem on the surface. So these four people don't just want to go on a pilgrimage and find the Fountain of Youth on a whim - there must be something important behind their decision.
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Based on the excerpt is that tom is clearly ignoring his aunt's remorse on purpose. It is stated that he knows that a "yearning glance" was upon him but he refused to recognize it that is to acknowledge it. He even goes so far to imagine denying his aunt what she wants to hear even on his deathbed.