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ASHA 777 [7]
2 years ago
13

Photosynthesis inputs

Biology
1 answer:
maria [59]2 years ago
3 0

Answer:

Light Intensity: Increased light intensity results in a higher rate of photosynthesis. On the other hand, low light intensity results in a lower rate of photosynthesis.

Explanation:

comment if you got question's

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Answer/Explanation:

(1) a mutation in the coding region, resulting in an inactive protein

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To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.

(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.

To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.

To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR

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