Question

In general, when recombination experiments are conducted with bacteria, participating bacteria are mixed in complete medium, then transferred to a minimal growth medium. Why isn't the protocol reversed: minimal medium first, complete medium second?

Answer 1

We can confirm that if we used a minimal medium first and attempted to transfer from that to a complete medium second, certain types of bacteria would not be able to survive the first medium.

What bacteria would be affected?

This has to do with many kinds of bacteria, but particularly auxotrophs. These are bacteria who have trouble producing the substances they need to survive, so in an incomplete medium, these bacteria would not have a high chance to survive until moved to the complete medium.

Therefore, we can confirm that if we used a minimal medium first and attempted to transfer from that to a complete medium second, certain types of bacteria would not be able to survive the first medium.

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