Chromatography is a simple technique in principle, it remains the most important method for the separation of mixtures into its components. It is quite versatile for it can be used to separate mixtures of solids.
Explanation:
- The two elements of chromatography are the stationary phase and the mobile phase. There are many choices of stationary phases, some being alumina, silica, and even paper. The mobile phase, in liquid chromatography, can also vary. It is often either a solvent or a mixture of solvents and is often referred to as the eluant.
- A careful choice of eluting solvent helps to make the separation more successful. The mixture is placed on the stationary phase. The eluant passes over the mixture and continues to pass through the stationary phase carrying along the components of the mixture.
- Chromatography is used in industrial processes to purify chemicals, test for trace amounts of substances, separate chiral compounds and test products for quality control. Chromatography is the physical process by which complex mixtures are separated or analyzed.
- Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase

Gown
The nurse would put on the gown first when utilizing personal protective equipment (PPE). The nurse would then put on the mask, goggles, and gloves.
<h3>What is the sequence followed while wearing a personal protective equipment (PPE)?</h3>
STEP 1: GOWN
- Cover the entire body, from the neck to the knees, the wrists to the end, and the back.
- Fasten around the waist and neck.
STEP 2: SHOE COVERS
- Layer shoes with shoe covers on top.
STEP 3: GLOVES
- Apply the first set of gloves and inspect them for tears.
- Thumb hole made in lab coat
- Thumb through opening in lab coat after pulling it over the hand
- Put on a second set of gloves (extend to cover wrist)
STEP 4: MASK OR RESPIRATOR
- As you cup the respirator in your hands, place the nosepiece there.
- With the nosepiece up, place the respirator under your chin. Pull the top strap over your head, putting it at the back of your head.
- Pull the bottom strap so that it is around your neck and just behind your ears.
- Mold the nose using two hands.
- Put both hands over the mask and take a forceful breath out.
- If air leaks, adjust the mask.
STEP 5: GOGGLES OR FACE SHIELD
- Put over face and eyes, then make adjustments to fit
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c. Light independent reactions
Answer:
<h3>Viruses can only replicate themselves by infecting a host cell and therefore cannot reproduce on their own.</h3>
<h3>At the most basic level, viruses consist of genetic material contained within a protective protein coat called a capsid; the existence of both genetic material and protein distinguishes them from other virus-like particles such as prions and viroids.</h3>
<h3>They infect a wide variety of organisms: both eukaryotes (animals, fungi and plants) and prokaryotes (bacteria).</h3>
<h3>A virus that infects bacteria is known as a bacteriophage, often shortened to phage.</h3>
<h3>The study of viruses is known as virology, and those who study viruses are known as virologists.</h3><h3 /><h3>It has been argued extensively whether viruses are living organisms.</h3>
<h3>Most virologists consider them non-living, as they do not meet all the criteria of the generally accepted definition of life.</h3>
<h3>They are similar to obligate intracellular parasites as they lack the means for self-reproduction outside a host cell, but unlike parasites, viruses are generally not considered to be true living organisms.</h3>
<h3>A primary reason is that viruses do not possess a cell membrane or metabolise on their own - characteristics of all living organisms.</h3>
<h3>Examples of common human diseases caused by viruses include the common cold, the flu, chickenpox and cold sores.</h3>