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Answer: It caused a mass migration of Native Americans from Georgia Eliminate.
Hernando de soto's forces were the first european troops that the natives ever seen, so they felt really bewildered when they first see De Soto's men.
After that, De Soto started to make conquests among this region which cause the native Americans to move out of this place in search of safety.
A trough is a low turning point or a local minimum of a business cycle. The time evolution of many variables of economics exhibit a wave like behaviour with local maxima (peaks) followed by local minima (troughs). A business cycle may be defined as the period between two consecutive peaks.
By 1600, Spain claimed it's territory on Centra, North and South America, and Europe. Hope it help!
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