Question

It is often desirable to express eukaryotic genes in bacteria, which can make a protein of interest quickly and cheaply. What is TRUE about this kind of genetic transformation

Answer 1

The expressed gene must be incorporated into the bacterial chromosome, but its promoter region should be replaced with a bacterial promoter.

What about bacterial chromosome?

• The term "nucleoid" refers to a bacterial chromosome.
• It has genetic material inside and is a strange shape. It may also be known as a genophore.
• Both transcriptional activators and repressors control gene expression in eukaryotic cells.

• Eukaryotic repressors bind to specific DNA sequences and prevent transcription, just like their prokaryotic counterparts.
• Since transcription and translation are physically distinct processes, eukaryotic gene expression is more complex than prokaryotic gene expression.
• Eukaryotic cells, as opposed to prokaryotic cells, have a wide range of levels at which they can control gene expression.
• While transcription and translation can both happen at the same time in bacteria, in eukaryotes, transcription takes place in the nucleus and translation happens in the cytoplasm.

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Question: It is often desirable to express eukaryotic genes in bacteria, which can make a protein of interest quickly and cheaply. What is TRUE about this kind of genetic transformation?

a. The expressed gene and the genes for all of its regulatory elements must be incorporated into the bacterial chromosome.

b. Only polycistronic genes are capable of being expressed in a bacteria.

c. The expressed gene and the gene for RNA Polymerase II must both be incorporated into the bacterial genome.

d. Only the transcribed region of the gene should be incorporated into the bacterial genome. No promoter is necessary to transcribe a transformed gene.

e. The expressed gene must be incorporated into the bacterial chromosome, but its promoter region should be replaced with a bacterial promoter.