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svetoff [14.1K]
2 years ago
8

Why is onpg used as the substrate for the β-galactosidase assay rather than lactose?

Biology
1 answer:
Colt1911 [192]2 years ago
7 0

Answer:

Explanation:

Unlike lactose, the substrate O-nitrophenyl-beta-D-galactopyranoside (ONPG) is capable of penetrating the bacterial cell without the presence of permease. In the broth method of testing, the organism is taken from a medium containing a high concentration of lactose and is inoculated into the ONPG Broth.

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