Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Crop monitoring makes use of space-based data to keep tabs on crop development and forecast crop yields for certain fields that have been planted.
<h3>What is monitoring systems?</h3>
A system for monitoring agriculture consists of a network with wireless sensors. These sensors gather information from several nodes positioned on the playing surface. Then, specialists or nearby farmers analyze this data. The data can be used to make a number of inferences about weather patterns, soil fertility, crop quality, etc. A system is developed for agricultural field monitoring in IoT-based modern agriculture with the aid of sensor like light, humid, temperatures, soil moisture, etc. Farmers may monitor the condition of thier fields from any location. IoT-based smart farming is considerably more efficient than conventional farming.
<h3>How do farmers monitor their crops?</h3>
Nowadays, satellite techniques are widely employed in agriculture, and many farmers use them frequently to observe their fields and assess the condition of their crops. Crop monitoring is crucial for managing various pests, weeds, and diseases that affect crops. This gives information about the crop's current situation, and you can then look ahead in time to forecast what will probably be the crop's next problem.
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The metabolism converts nutrients into energy or materials for growth, reproduction, repair, and maintenance.
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The answer is nucleic acid