Question

We used the enzyme, taq polymerase, which is derived from the thermus aquaticus bacterium, in our pcr procedure. Why is this enzyme important for efficiency in pcr?.

Answer 1

The PCR amplification process most frequently employs an enzyme known as Taq DNA polymerase.

Taq Polymerase, or Taq DNA  polymerase, is a biological catalyst that helps nucleotides join together to form DNA. Taq polymerase is a protein that is 832-amino acids long and has a molecular weight of 94 kilodaltons. It is a homolog of the Pol I DNA polymerase that is found in Escherichia coli (E.coli) (approx). This enzyme can withstand temperatures up to 95 degrees Celsius for 40 minutes before reaching its half-life. At the ideal temperature of 72 degrees Celsius, the rate of nucleotide incorporation ranges from two to four kilobases per minute.  

Polymerase chain reaction operates at a very high temperature. At this temperature, Taq polymerase is able to carry out its function efficiently.

Learn more about polymerase chain reaction here :

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