A easy DNA stretching technique for fluorescence imaging of unmarried DNA molecules
Stretching or aligning DNA molecules onto a surface through molecular combing techniques is one of the important steps in unmarried DNA molecule evaluation. however, a few of the modern-day research have focused on lambda-DNA, or different big DNA molecules.
There are only a few studies on stretching methodologies for DNA molecules generated thru PCR (generally smaller than 20 kb). right here we describe a simple technique of stretching DNA molecules as much as 18 kb in length on a modified glass floor. The very low background fluorescence permits green detection of unmarried fluorescent dye labels incorporated into the stretched DNA molecules.
Stretching or aligning DNA molecules onto a surface by using molecular combing strategies is one of the critical steps in unmarried DNA molecule analysis. however, the various current studies have targeted on λ-DNA, or different huge DNA molecules
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