Only the culturable organisms in the sample, which can represent as little as 1% of the total bacteria present, are indicated by the heterotrophic plate count.
<h3>What is heterotrophic pate count technique?</h3>
A variety of factors can influence how bacteria grow on a given media. These variables include the make-up of the incubation medium, incubation temperature, incubation period, etc. Fastidious bacteria, for example, are unable to grow on the Nutrient Agar medium, and some germs can take a very long period to form colonies that are visible.
The actual number of microorganisms in the sample may be significantly higher than that estimated by a heterotrophic plate count, for the two primary reasons mentioned above.
Thus, Only the culturable organisms in the sample, which can represent as little as 1% of the total bacteria
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