The survival or neuronal development of human embryonic or human induced pluripotent stem cells is not improved by feeding them more than five days per week.
<h3>Why does feeding not increase neuronal differentiation or cell survival?</h3>
An induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) can differentiate into any form of specialized human cell, ESC/iPSC-derived cell types have a lot of potential for use in regenerative medicine.
Neuroscience research can benefit from neural culture of human pluripotent stem cells. However, it is unknown what the ideal feeding schedule for these in vitro systems should be. Human embryonic and induced pluripotent stem cells were cultivated with medium exchange schedules of five, six, or seven days per week during two months of differentiation. We assessed the survival and neural differentiation characteristics of these cells. Through this period of culture, neither human pluripotent cell type showed any appreciable alterations in cell counts or indicators of brain development. We come to the conclusion that feeding this culture system more than five days a week is unlikely to be advantageous.
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