Question

comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability

Answer 1

To identify, map, and evaluate the underlying biochemical processes of cell signaling, it is essential to visualize cells and cellular structures as well as track and modify nucleic acids and proteins in living cells.

How cell labeling of mammalian cells done?

• Mammalian cells can be magnetically labeled by combining ferumoxides or superparamagnetic iron oxide (SPIO) with transfection agents (TAs).
• The effectiveness and survivability of cell labeling with TAs complexed to SPIO have not been systematically compared. This study examines the toxicity and labeling effectiveness of FEs complexed to various TAs in mammalian cells at various doses.
• TAs from several classes, including lipid-based substances, dendrimers, and polycationic amines, were employed.
• TAs at concentrations ranging from 1 to 50 microg/mL in incubation media were used to measure the cellular toxicity. Combining varying doses of TAs with various amounts of FEs allowed researchers to calculate the iron incorporation efficiency.

Learn more about the cell labeling with the help of the given link:

brainly.com/question/16758407

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