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Vedmedyk [2.9K]
3 years ago
11

The use of restriction enzymes and electrophoresis to generate a map of the DNA molecule is known as

Biology
1 answer:
Lerok [7]3 years ago
7 0

The use of restriction enzymes and electrophoresis to generate a map of the DNA molecule is known as RESTRICTION MAPPING. This technique is used to map an unknown fragment of DNA.

Restriction enzymes are enzymes that cut a DNA fragment at specific nucleotides sites, thereby generally smaller fragments of DNA.

Restriction mapping is a strategy used to map unknown fragments of DNA by digesting them into smaller fragments and then identifying the specific sites of the DNA breakpoints.

Restriction mapping can then be used to create a diagram of fragments of DNA marked with specific sites where they are cut by specific restriction enzymes.

Learn more in:

brainly.com/question/1127662

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The numbers are 2 of them
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What type and gauge of suture would be used to anastomose a synthetic aortic graft onto an aorta during an abdominal aortic aneu
ikadub [295]

Answer:

Explanation:

There are several approaches and surgical techniques in the reparation of an abdominal aortic aneurysmectomy according to the recent studies. In certain cases, it is recommended to use standard dacron graft with a flexible collar (the collar graft). The other one applies standard dacron graft.

Abdominal aortic aneurysm (AAA) is linked to 1.3% mortality in the male population older than 65-85 years. AAA is defined as the degradation of the elastic media of the previously atheromatous aortic wall. AAA occurs between the aortic hiatus in the diaphragm (T12 vertebra level) and the bifurcation into the common iliac arteries (L4 level). The diameter of the normal aorta varies in the population. In men, it is between 16 to 22 mm.

The most common risk factors for the AAA developing are smoking, male sex, older age, hypertension, hyperlipidemia and previous vascular disease.  

The most common symptoms of AAA are a pain in the abdomen, chest or lower back. The presence of peripheral vascular disease if thrombus formation is present within the aneurysm or mural emboli decrease blood flow to the periphery. In certain individuals, there is a ureteric obstruction due to the compression. The diagnosis is performed by bimanual palpation in the region above the umbilicus. Other diagnostic imaging is necessary most commonly via ultrasound and  CT scan.

Open repair is performed under general anaesthesia. The abdominal cavity is entered via a midline incision, the AAA is detected. Proximal control is provided with the clamp to the aorta (below the renal arteries). The distal control is provided via clamping the common iliac arteries. The aneurysm is opened and any thrombus removed. A graft is anastomosed to either end of the affected region of the aorta. Thus, clamps are removed and blood flow is maintained.

EVAR repair is performed by the stent-graft placement within the aneurysm which requires CT angiography preoperatively. The graft has 2 components, of which both are inserted on catheters under fluoroscopic guidance. EVAR is performed under local anaesthesia with sedation, epidural or spinal block.

The main body of the graft is inserted via the common femoral artery, while the contralateral is then inserted via the other femoral artery. In this way, there is an entirely new lumen for the distal aorta and proximal common iliac arteries.

In the case of an endovascular repair of abdominal aortic aneurysms, total percutaneous access or endovascular aortic aneurysm (EVAR) has been documented. In this approach, the most common material used may be constructed from self-expanding nickel-titanium (nitinol) with polyester graft material. The complications are defined as suture break (separation of adjacent metallic rings) and metal-ring fracture.

The most common postoperative complications are pseudoaneurysm, haematoma,  graft-enteric fistula, suture line disruption.  In case of open abdominal repair, there is always a possibility for a hernia complication and connective tissue disorder.

5 0
3 years ago
Read 2 more answers
A transcriptional repressor that controls the transcription of gene A is not normally active unless bound by an effector molecul
vodomira [7]

Answer:

Transcription of gene A is not affected

Explanation:

A transcriptional repressor is a DNA/RNA-binding protein that acts to suppress gene expression and/or protein synthesis by binding to target sequences. Moreover, a silencer is a DNA region that binds to particular transcriptional repressors in order to suppress gene expression. In this case, the transcriptional repressor is activated by binding to an effector molecule X (e.g., another protein). In consequence, and since the activated repressor-X complex is not more able to bind the silencer of gene A, it is expected that such complex will not be able to affect transcription of the target gene.

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Which feature distinguishes slime molds from fungi?
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Answer:

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3 0
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12)Scientists study the biosphere to examine the relationship between biotic and abiotic factors that impact the entire planet a
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Answer:D

Explanation:

This isn’t B, because it’s only one species. It isn’t C, because it is one population, and A doesn’t make sense due to how they are of course organisms. D is your best bet.

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