P site, initiation step of translation, the fmet charged tRNA assembles in this site of the ribosome.The small ribosomal subunit, mRNA, initiator tRNA, and large ribosomal subunit come together to form the initiation complex. The P site of the completed ribosome is where the initiator tRNA is located.
When an amino acid, tRNA, and mRNA come together inside the ribosome, translation begins. The process of translation continues as the mRNA passes through the ribosome after it has started. Each codon pairs with a new tRNA anticodon, bringing in new amino acid to lengthen the chain.
At the subunit interface, tRNA molecules bind to the ribosome in a solvent-accessible channel. The big and small subunits each feature three tRNA binding sites known as the aminoacyl site (A site), peptidyl site (P site), and exit site (E site). The expanding polypeptide chain of amino acids is held in place by the tRNA by the P site, also known as the peptidyl site. The new amino acid that will be added to the polypeptide chain is stored in the aminoacyl tRNA, to which the A site (acceptor site) binds.
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