Following hybridization, the micro array is scanned to measure the expression of each gene printed on the slide. If the expression of a particular gene is higher in the experimental sample than in the reference sample, then the corresponding spot on the micro array appears red.
What kind of data is generated when micro array experiments are visualized?
Micro array experiments generate vast quantities of raw gene expression data, therefore good experimental design and statistical analysis is required for the extraction of accurate and useful information regarding the expression of genes.
What conclusions can be made from a DNA microarray?
Microarray data allows you to determine the level of gene expression in a patient for many genes at one time. In this case, the data provides some insight into which genes may be affected in the development of cancer.
Steps involved in cDNA based microarray:
- Creation of labeled cDNA.
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