The Northern blot technique uses DNA, antibody, RNA, probes, and proteins to identify biological molecules.
<h3>A Northern blot reveals what?</h3>
RNA molecules can be identified and measured via northern blot analysis. Northern blot analysis, in contrast to techniques like reverse transcriptase PCR (RT-PCR) or RNase protective assay, may identify RNA molecules by their size.
<h3>What is the Northern blotting principle?</h3>
The fundamental idea behind Northern blotting is the separation of RNA into different sizes, which is then detected on a membranes using a hybridized probe with a template strand complementary to all or a portion of the sequences of the target mRNA. RNA, DNA, or oligodeoxynucleotide probes that are radioactively or non-radioactively tagged can be used to examine Northern blots.
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