Question

C-banding is a pattern of staining using giemsa stain in which chromosomes show staining of?

Answer 1

Giemsa stain shows the staining of centromeres.

• C-banding, which involves denaturing the chromosomes in a saturated alkaline solution followed by Giemsa staining, is a technique specifically used to identify heterochromatin.
• Different banding methods can be selected when identifying chromosomes. Chromosome banding is an important technique used in chromosome karyotyping to distinguish between normal and defective chromosomes for clinical and scientific purposes.
• The most common dye-based chromosome banding methods are Giemsa (G), reverse (R), and centromere (C). G-banding stains trypsinized chromosomes, whereas R-banding uses hot acidic saline to denature the chromosomes prior to Giemsa staining.
• It contains a significant amount of AT-rich satellite DNA. Just as G-banding represents AT-rich sections of chromosomes, so does Q-banding.

Therefore, centromeres is the correct answer.

Learn more about centromeres:

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