1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Luden [163]
1 year ago
5

proteins need to be denatured using an anionic detergent such as sds before gel electrophoresis because: select all that apply.

Biology
1 answer:
grandymaker [24]1 year ago
8 0

The majority of proteins contain a uniform positive charge, and a uniform neutral charge, and most proteins have intricate three-dimensional structures that might affect how well they travel through the gel electrophoresis. For these reasons, proteins need to be denatured. Here, the right answers are A, B, and D.

Protein separation is accomplished using a molecular technique called sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which operates in the proximity of an electric charge. Proteins are separated using SDS-PAGE according to their electrophoretic mobility. Protein charge, shape, and structure all affect electrophoretic mobility.

To proceed in the direction of the positive terminal, the protein molecules need to have a constant negative charge (anode). The overall charge of a protein is determined by the amino acid content of the protein as well as the charge of the individual amino acids.

Thus, depending on the total makeup of amino acids, the charge may change. Depending on the ratio of different amino acids, the total charge of the proteins can be positive, negative, or neutral. The SDS treatment gives the proteins a consistent, negative charge. Then, according to size, the negatively charged amino acids flow toward the positive terminal.

Complete question:

Proteins need to be denatured using an anionic detergent such as SDS before gel electrophoresis because: Select all that apply.

A - most proteins have a uniform positive charge

B - most proteins have complex three-dimensional structures that can impact their movement through the gel

C - the overall charge of a protein depends on the amino acid composition of the protein and can vary greatly

D - most proteins have a uniform neutral charge

To learn more about gel electrophoresis

brainly.com/question/9437877

#SPJ4

You might be interested in
The cell membrane controls the traffic of molecules into and out of the cell, prevents the entry of toxic material into the cell
elena55 [62]
"<span>Semi-permeable nature of the membrane" is the property of the membrane among the choices given in the question that this can be attributed to. The correct option among all the options that are given in the question is the fourth option or option "D". I hope that the answer has actually come to your help.</span>
7 0
3 years ago
Read 2 more answers
In 6-8 sentences, explain how stream volume and speed affect the size, shape, and
ki77a [65]

Answer:

Stream volume and speed directly affects the size, shape, and  flow of streams and rivers.

Explanation:

  • The greater volume results in larger channels and these larger channels will make the flow of stream faster.
  • The flow volume of different streams are related to precipitation and run-off.
  • The earth materials also heavily effect the flow by changing the properties of the water like increase in the mass, hardness, shape, etc.
  • Erosion and deposition is common in the curving type of stream.
5 0
3 years ago
Anchoveta feed on large
mojhsa [17]

Answer:

Anchoveta feed on large  zooplankton whereas sardines  feed mainly on phytoplankton.  Use this information to suggest  why sardine numbers are less

likely to collapse during an El  Niño event?

Reason why sardine are less likely to collapse during an El Nino event is as  a result of sardine survival is neither dependent  on zooplankton nor anchoveta rather sardine is dependent on phytoplankton which is not scarce

Explanation:

8 0
3 years ago
the substance that are before any chemical reaction are called 1) dioxides 2) oxygen 3) products 4) reactants
Vesna [10]
The answer is Reactants :)


6 0
3 years ago
What type of cell is a rose thorn
barxatty [35]

rose thorns are multicellular

i hope this helps! :)

3 0
3 years ago
Read 2 more answers
Other questions:
  • - Which of the following is an interaction in which<br> both organisms are harmed
    11·2 answers
  • Explain why a satellite in polar orbit can take pictures of all parts of the Earth over time.
    8·1 answer
  • Will someone help me do my biology vocab for 45 points?
    14·1 answer
  • Remnants of erythrocyte nuclei, nuclear fragments, or aggregates of chromosomes are called:
    14·1 answer
  • What happens when planaria grow, heal and regenerate after being cut
    14·1 answer
  • Suppose a family replaces ten 60-watt incandescent bulbs with ten 30-watt fluorescent lamps. If each light was used for 4 hours
    8·1 answer
  • How does geoscience processes and current human activities affect resources in a swamp?
    7·1 answer
  • Why do some scientists now argue a pushing, not a pulling, is behind the Atlantic’s slow expansion?
    11·1 answer
  • Genetic alteration probably refers to altering what…. _____________________________ (found in the nucleus)
    10·2 answers
  • A biology class is studying the concept of carrying capacity. On a field trip, the
    11·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!