Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer:
Potential & Kinetic energy.
Explanation:
The water used gains potential energy overtime before it is converted to mechanical (or kinetic energy). This can be explained by the water having increased potential energy as it flows at an equal altitude, but converting to kinetic energy as it flows downhill, or over the dam.
This kinetic (or mechanical) energy is then converted to traditional electricity. This process is also an example of a renewable resource being utilized for everyday things (such as powering cars, homes. or charging batteries).
Answer:
there is no image attachment. if u add one, i can help!
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Explanation:
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~mina
Fertilization and formation of the zygote occur Within the body of mosquito.
Zygote, from the Ancient Greek (zygtós), "attached, yoked," from (zygoun), "to join, to yoke," A fertilization between two gametes produces a eukaryotic cell. The genome of a zygote, which consists of the DNA from each gamete, is what makes up a new individual creature and holds all of its genetic material.
The zygote is the first developmental stage in animals with many cells. When an egg cell and sperm cell unite to produce a new, distinct organism, a zygote is created in humans and the majority of other anisogamous species. With the aid of mitosis, the zygote can divide asexually in single-celled organisms to create identical progeny. The chloroplast DNA (cpDNA) of a Chlamydomonas zygote is inherited uniparentally from the parent with the mt+ mating type; as a result, such cells are typically uncommon. The mapping of chloroplast genetics through recombination was made possible by these uncommon biparental zygotes.
Learn more about Zygote here:
brainly.com/question/465851
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Producers,consumers,and decomposers are linked because they all have to work together to stay alive the produces will make the energy inside itself then the consumers will eat it then have all the energy the plant had then the consumer will die and the decomposer will beak it down and eat what remains of the animal
i hoped this helped :)
