The best and most correct answer among the choices provided by your question is The best and most correct answer among the choices provided by your question is the last choice.
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Kingdom Protista is not valid under evolutionary classification because they evolved before any other kingdom, and as such share a most recent common ancestor with each other, as opposed to any other group.</span>
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The oil extracted through these methods is referred to as "green crude" and it's not ready to be used as fuel until it undergoes another process called transesterification. This step adds more substances to the mix, including alcohol and a chemical catalyst that causes the alcohol to react with the oil. This reaction creates a mix of biodiesel and glycerol. The final step in processing separates the glycerol from the mixture and leaves a biodiesel that's ready to be used as fuel. Maybe one day it really will be easy being green.
You can read more about it here https://auto.howstuffworks.com/fuel-efficiency/biofuels/convert-algae-to-biofuel.htm
It becomes positive because ATP synthase allows H+ ions to pass through the membrane .
