There are many reasons to why slaves were essential in the colonial economy:
Slaves were generally used more in the south, because large plantations were found there, which meant that the need for large amounts of workers.
Slaves provided cheap labors, and as they were not "citizens" of the US, they were not allowed their rights.
Slaves were also relatively easy to buy and replace, however, their survival rates were not that good after they traveled long distance.
etc.
hope this helps
Answer:
The promoter region/sequence
Explanation:
Promoter sequences/region is where the transcription factors and RNA polymerase must attach before the gene can be transcribed. SO if we mutate this sequence, the transcription factors and RNA polymerase won't recognize it thus would not attach and transcribe the targeted gene.