1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
ludmilkaskok [199]
3 years ago
15

To study the molecular mechanism of DNA replication, you incubated soluble E. coli extracts with a mixture of dATP, dTTP, dGTP,

and dCTP, all of which are labeled with the -phosphate group. After a while, the incubation mixture was treated with trichloroacetic acid, which precipitate the DNA but not the nucleotide or very short oligonucleotides. The precipitate was collected and the extent of the radioactively label nucleotide incorporation into the DNA was determined.
a.) If any one of the four nucleotides is omitted from the incubation mixture, would you detect radioactivity in the precipitate? Explainb.) Would 32P be incorporated into the DNA if only dTTP is labeled? Explainc.) Would radioactivity be found in the precipitate if 32P labeled the β and γ rather than the α phosphate of the deoxyribonucleotides? Explain.
Biology
1 answer:
Hoochie [10]3 years ago
8 0

Answer:

Explanation:

A) Since, mixture of dATP, dTTP, dGTP, and dCTP all were labeled; radioactivity can be detected even if one of the four nucleotides is omitted from the incubation mixture. This is because all other three nucleotides contain labeled phosphate and hence, radioactivity can be detected.

B) Yes. If 32P is labeled only dTTP, it can be detected in DNA. Since, dTTP can pair with 'A' nucleotide in DNA.

C) Mostly radioactivity can be detected in DNA when the alpha or terminal phosphates are labeled. It is difficult to detect radioactivity when phosphate groups other alpha like beta or gamma phosphates are labeled. Even if the radioactivity is detected it can be detected in rare cases like 5`end labeling of DNA on gamma phosphate.

You might be interested in
Steroids are a major class of____.
Gennadij [26K]

Answer:

D

Explanation:

Additional classes of steroids include:  

Neurosteroids such as DHEA and allopregnanolone

Aminosteroid neuromuscular blocking agents such as pancuronium bromide

6 0
3 years ago
When a player steps into his/her backswing, where should the weight of his/her body be placed? (1 point) le???? hip right hip ba
Maru [420]
<span>I am going to answer this as a golf player myself
  The answer to this question is it is rather a chain of event where where weight shift occurs gradually while back swing but the weight is originally on the back foot and for good back swing the weight should be on it so that more power can be delivered while swinging So the correct answer is back foot</span>
8 0
3 years ago
Proteins have a variety of functions within a living cell. Describe at least three of the possible functions of proteins, and ex
lidiya [134]
<span> 
Q:</span><span>Proteins have a variety of functions within a living cell. Describe at least three of the possible functions of proteins, and explain how proteins can be</span><span> 

A:Any of a group of complex organic macromolecules that contain carbon, hydrogen, oxygen, nitrogen, and usually sulfur and are composed of one or more chains of amino acids. Proteins are fundamental components of all living cells and include many substances, such as enzymes, hormones, and antibodies, that are necessary for the proper functioning of an organism. They are essential in the diet of animals for the growth and repair of tissue and can be obtained from foods such as meat, fish, eggs, milk, and legumes.</span>
7 0
3 years ago
Read 2 more answers
What is a force that attracts all matter to each other
Ganezh [65]
The force that attracts all matter to each other is gravity, i believe.
3 0
3 years ago
Read 2 more answers
What happens when energy is transferred to a liquid? If you really know the answer from previous lessons like this, then I will
Ierofanga [76]

Answer:

Convection occurs when particles with a lot of heat energy in a liquid or gas move and take the place of particles with less heat energy. Heat energy is transferred from hot places to cooler places by convection. Liquids and gases expand when they are heated. ... The denser cold liquid or gas falls into the warm areas.

4 0
3 years ago
Read 2 more answers
Other questions:
  • Which can change from one state of matter to another at the temperatures and pressures experienced at earth’s surface?
    6·2 answers
  • What distinguishes the savanna and grassland biomes?
    15·1 answer
  • A cross between homozygous red-eyed flies and homozygous white-eyed flies results in progeny that all have red eyes. this result
    8·1 answer
  • Bratz babyz fish tank how long does it take for eggs to hatch
    5·1 answer
  • What is the basic cause of ocean tides? A. Gravitational attraction between the Moon and the entire solar system B. Gravitationa
    6·2 answers
  • Which of the following processes causes the release of nitrogen back in to the atmosphere
    9·1 answer
  • What is the name of the lab tool that is used to transfer a sample to a microscope slide?
    5·2 answers
  • Hello everyone please help with these three questions, thanks in advance! 1. What is the difference between a genotype and a phe
    7·2 answers
  • Which phrases describe groundwater? Check all that apply.
    14·2 answers
  • 47
    7·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!