No. At that point, you need to determine if the problem lies in the control or the staining reagents or techniques.
Pressure and heat is the two forces
Answer:
this mutation may change the open reading frame of the resulting RNA sequence and its final product, which is a protein in the case that this gene is used to synthesize a messenger RNA (mRNA) sequence
Explanation:
During the transcription, a region of DNA named 'gene' is used as template to produce an RNA molecule, typically a primary transcript of mRNA (pre-mRNA). Subsequently, this pre-mRNA suffers a process named RNA processing in order to generate a mature mRNA which is finally used to create a protein by a process called translation. If a deletion occurs during transcription, it may change the open reading frame (ORF) of the resulting mRNA when the mutation occurs in an exon of the protein-coding gene (i.e., occurs a frameshift mutation), while this deletion may not have any effect if it is localized within the introns which are removed during RNA processing. A frameshift mutation will change the amino acids that are added to the nascent polypeptide chain during translation.
Answer:
First, you need to retrieve the FASTA sequences of the already sequenced genes. After that, you should make a sequence alignment on a software like CLUSTALW (https://www.ebi.ac.uk/Tools/msa/clustalw2/) to seek for the conserved regions of the gene. Based on this regions, you will design your FISH probe, which for bacteria can start in 33 bp (Ming Tan <em>et al.</em>, 2019), but a 200 bp probe is a very suitable size. There are several companies that fabricates this custom probes, and sell all the reagents to perform the experiments. Having this, you will perform your FISH experiment to see which cells are actively degrading Toluene.
Explanation:
