Biotic Factors depend/rely on abiotic factors because living things (biotic) need nonliving things (abiotic) to survive. Biotic rely on other Biotic factors because they need the food and resources from other living things.
For example, we (biotic factors) need energy from the sun and oxygen/air ,which is abiotic factors, to live and grow. Just like plants (biotic) need the sun's energy (abiotic) to help it grow.
I hope u understand me. Sorry If u don't
Because only the chromosomes can be seen in a karyotype, and microdeletions or insertions are mutations at the molecular level, it is virtually impossible to detect such mutations at the chromosomal level.
To be honest I don’t really know
Just think about cancer.. it's uncontrolled cell growth, so mutations in genes can cause cancer by accelerating cell division. As the cells contribute to grow they then develop as a tumor.