Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
Louis Pasteur was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination, microbial fermentation, and pasteurization.
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Answer:
The correct answer is -
1. right-handed
2. right-handed
3. positive
4. left-handed
5. negative
6. negative
Explanation:
In a circular bacterial chromosome, the structure of DNA is a right-handed double helix In a circular bacterial chromosome.
If DNA is twisted in the right-handed direction, it becomes overwound. Overwinding results in positive supercoiling takes place. If DNA is twisted in the left handd direction, it becomes underwound. Underwinding results in negative supercoiling.
f. One effect negative supercoiling in bacterial chromosomes is to promote separation of the two strands of DNA in the double helix
Answer:
It's option A- Solid
Explanation:
Solids have definite shape and volume.
