is that the full question?
Answer:
Elements. Metals. Ionic compounds, such as salt
Explanation:
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The purine or pyrimidine base was removed from the labeled nucleotide base.
A DNA polymerase enzyme recognizes this and replaces the is inserted nucleotide allowing replication to continue. Calibration corrects approximately 99% of these types of errors but is still not sufficient for normal cellular function. Topoisomerases act in the region before the replication fork and prevent supercoiling.
Primase synthesizes RNA primers complementary to the DNA strand. Cells have various mechanisms to prevent mutations and permanent changes in DNA sequences. During DNA synthesis, most DNA polymerases check their work by repairing most mismatched bases in a process called proofreading. Errors can occur during the process of DNA replication. Nucleotide bases can be is inserted, deleted, or mismatched in a DNA strand.
Learn more about Nucleotides here:-brainly.com/question/1569358
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Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
Why did he??????
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