Question

Carbolfuchsin used in the original acid fast stain consisted of 0.3 g basic Fuchs in 10 ml 95% ethanol 5ml phenol and 95 ml water . The recipe for the kingpin modification is 4 g basic fuchsin in 20 ml ethanol 8g phenol and 100 ml water . The original acid fast stain require heating the smear to force the carbolfuchsin into the wall. Why can heat be eliminated in the kingpin modification that you used ?

Answer 1

MycolicWhat are the components of acid alcohol?Prevent contamination of the smear by the stain pptWhat is the purpose of placing a filter paper on the glass slide?1. Flood with carbolfuchsin. Heat until steam is given off for about 5 min
2. Allow to stand for more 5 min w/o heating. Remove the filter paper
3. Decolorize with acid alcohol while continously agitating the slide, then wash with water
4. Counterstain with methylen blue for 30 sec., wash with water then let it dryDiscuss the procedure in using ziehl-neelsen methodRedWhat is the color of an acid fast bacteria?Blue/greenWhat is the color of non-acid fast bacteria?4g basic fuchsin
20 ml of 95% ethanol
8 ml phenol
100 ml distilled waterWhat are the components of kinyoun's carbolfuchsin?1. Flood the smear with kinyoun's for about 5.min 
2. Rinse with deionized water
3. Decolorize to faint pink color with acid alcohol for 3 min
4. Rinse again with deionized water
5. Flood with methylene blue for (4min), dry and examine under OIODiscuss the procedure in using kinyoun's methodHorizontal or vertical scanningWhat is the proper way or direction of reading your stained smear?Horizontal scanningWhat is known as standard scanning?Pappenheims methodIn what method we use rosalic acid and alcohol as decolorizer?ColorlessWhat is the reaction of Mycobacterium smegmantis when rosalic acid and alcohol was added as decolorizer in pappenheims method?Diluted alcholic fuchsinWhat is use as stain i youe baumgarten's method?Red

Answer 2

Answer:

The reason is that, the mycobacterium wax-like lipid such as Nocardia is not susceptible to staining or decolorization, hence, the heat allowed penetration of the stain. Also, the kinyoun modification does not require heat because a more concentrated fuchsin is used

Explanation:

The Kinyoun method or stain, otherwise known as cold method, is a procedure used to stain acid-fast species of the bacterial genera Mycobacterium and Nocardia and the apicomplexan genus Cryptosporidium.

Developed by Joseph Kinyoun, researched that, given particular species of bacteria that have a waxy lipid called mycolic acid, in their cell walls, this allow them to be stained with Acid-Fast better than a Gram-Stain.

This is due to the fact that mycobacteria, has the special ability to resist staining or decolorization byacid-alcohol, hence, it was termed acid-fast.

The procedure involves the application of a primary stain (basic fuchsin), a decolorizer (acid-alcohol), and a counterstain

Therefore, in this case, the reason the heat can be eliminated in the kingpin modification is that, the mycobacterium wax-like lipid such as Nocardia is not susceptible to staining or decolorization, hence, the heat allowed penetration of the stain. Also, the kinyoun modification does not require heat because a more concentrated fuchsin is used.