1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Daniel [21]
3 years ago
14

Carbolfuchsin used in the original acid fast stain consisted of 0.3 g basic Fuchs in 10 ml 95% ethanol 5ml phenol and 95 ml wate

r . The recipe for the kingpin modification is 4 g basic fuchsin in 20 ml ethanol 8g phenol and 100 ml water . The original acid fast stain require heating the smear to force the carbolfuchsin into the wall. Why can heat be eliminated in the kingpin modification that you used ?
Biology
2 answers:
AysviL [449]3 years ago
8 0
MycolicWhat are the components of acid alcohol?Prevent contamination of the smear by the stain pptWhat is the purpose of placing a filter paper on the glass slide?<span>1. Flood with carbolfuchsin. Heat until steam is given off for about 5 min
2. Allow to stand for more 5 min w/o heating. Remove the filter paper
3. Decolorize with acid alcohol while continously agitating the slide, then wash with water
4. Counterstain with methylen blue for 30 sec., wash with water then let it dry</span>Discuss the procedure in using ziehl-neelsen methodRedWhat is the color of an acid fast bacteria?Blue/greenWhat is the color of non-acid fast bacteria?<span>4g basic fuchsin
20 ml of 95% ethanol
8 ml phenol
100 ml distilled water</span>What are the components of kinyoun's carbolfuchsin?<span>1. Flood the smear with kinyoun's for about 5.min 
2. Rinse with deionized water
3. Decolorize to faint pink color with acid alcohol for 3 min
4. Rinse again with deionized water
5. Flood with methylene blue for (4min), dry and examine under OIO</span>Discuss the procedure in using kinyoun's methodHorizontal or vertical scanningWhat is the proper way or direction of reading your stained smear?Horizontal scanningWhat is known as standard scanning?Pappenheims methodIn what method we use rosalic acid and alcohol as decolorizer?ColorlessWhat is the reaction of Mycobacterium smegmantis when rosalic acid and alcohol was added as decolorizer in pappenheims method?Diluted alcholic fuchsinWhat is use as stain i youe baumgarten's method?Red
Rudiy273 years ago
8 0

Answer:

The reason is that, the mycobacterium wax-like lipid such as Nocardia is not susceptible to staining or decolorization, hence, the heat allowed penetration of the stain. Also, the kinyoun modification does not require heat because a more concentrated fuchsin is used

Explanation:

The Kinyoun method or stain, otherwise known as cold method, is a procedure used to stain acid-fast species of the bacterial genera Mycobacterium and Nocardia and the apicomplexan genus Cryptosporidium.

Developed by Joseph Kinyoun, researched that, given particular species of bacteria that have a waxy lipid called mycolic acid, in their cell walls, this allow them to be stained with Acid-Fast better than a Gram-Stain.

This is due to the fact that mycobacteria, has the special ability to resist staining or decolorization byacid-alcohol, hence, it was termed acid-fast.

The procedure involves the application of a primary stain (basic fuchsin), a decolorizer (acid-alcohol), and a counterstain

Therefore, in this case, the reason the heat can be eliminated in the kingpin modification is that, the mycobacterium wax-like lipid such as Nocardia is not susceptible to staining or decolorization, hence, the heat allowed penetration of the stain. Also, the kinyoun modification does not require heat because a more concentrated fuchsin is used.

You might be interested in
Is there any evidence to prove that the earth is spherical​
Licemer1 [7]

Answer:

physics.

Explanation:

8 0
3 years ago
Read 2 more answers
Name the bacteria found in the root nodules of leguminuos plant​
sveticcg [70]

Answer:

Rhizobium is a genus of bacteria associated with the formation of root nodules on plants. These bacteria live in symbiosis with legumes.

Explanation:

4 0
2 years ago
Reflex activity can be modified by the cerebral cortex. For example, you are baking a roast in the oven. As you are moving the r
Zanzabum

Answer:

There are different types of reflexes, and some of them can be controlled voluntarily due to cortical processing.

Explanation:

Even if there is an initial myotactic reflex of moving away from the heat, the cortical processing that is subject to learning makes you not let go of the pan. It should be noted that there are different types of reflexes, and not all postural reactions are automatic. The cerebral cortex influences voluntary postural adjustment movements. Such control is mainly performed by the brain stem, where ocurrs the modulation of motor neurons and spinal cord interneurons through the lateral descending (fine movements) and medial (posture and balance) downward pathways.

7 0
3 years ago
This is a compound made up of carbon hydrogen and oxygen atoms it is used by cells to store and release energy sugars are made b
Sladkaya [172]

The answer is; glucose


It is a 6 carbon ring simple sugar and is the most abundant monosaccharide in the body. Its levels in the body are referred to as blood sugars. Larger carbohydrates consumed in the diet are broken down to glucose utilized in respiration (Glycolysis and Krebs cycles) to produce the energy required by the cells. Plants, on the other hand, manufacture carbohydrates in photosynthesis.


5 0
3 years ago
An igneous rock contains
Morgarella [4.7K]
These minerals are quartz, feldspar, mica, and usually hornblende. Granite forms as magma cools far under the earth's surface. Because it hardens deep underground it cools very slowly. This allows crystals of the four minerals to grow large enou
6 0
3 years ago
Other questions:
  • Describe rhe major functions of of each connective tissue
    8·2 answers
  • Why does the human Gene function properly in mice
    11·1 answer
  • 1. In which set is a biotic factor paired with an abiotic factor?
    10·2 answers
  • How do plants make lipids
    13·1 answer
  • One trait a plant can have is either red leaves (R) or brown leaves (r). Another trait is tall stems (T) or short stems (t). A p
    15·1 answer
  • In his study on canine cognition, Sherman guesses that German shepherds are more likely to find hidden objects than other breeds
    12·1 answer
  • A population of fruit flies on an island lay their eggs in some rotting fruit. Some of the fruit is blown into the ocean by a st
    9·1 answer
  • (a) The consequences of a growing human population have been a concern since the times of Thomas Malthus when he proposed that h
    12·1 answer
  • Define one kg mass , one meter , one second time <br>​
    7·1 answer
  • Record the growth of the organisms when the water temperature averages 30°?
    13·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!