Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
I don't know the question u are trying to say
Answer:
The relationship between photosynthesis and cellular respiration is such that the products of one system are the reactants of the other.
Explanation:
For example, the output of photosynthesis is the input of glucose production or cellular respiration. The output of glucose production is the input of photosynthesis. Photosynthesis involves the use of energy from sunlight, water, and carbon dioxide to produce glucose and oxygen. Cellular respiration uses glucose and oxygen to produce carbon dioxide and water. To emphasize this point, even more, the equation for photosynthesis is the opposite of cellular respiration.
The gene which is produced once or twice in a lifetime is the milk producing hormone after childbirth. This is because it is produced only after the birth of the child. The growth of the mammary glands takes place during pregnancy. The breast continue to increase in size after giving birth to a child. After delivery only copious milk is produced which is generally done with the help of milk hormone. During pregnancy the secretion of the milk is inhibited by the estrogen and progesterone which circulates in the blood stream which in turn inhibits the release of prolactin from the pituitary gland thus making the mammary glands unresponsive. Where as insulin is a hormone which helps the body to utilise the sugar or glucose from different sources like carbohydrates that we get while we eat food and adrenaline is secreted when we are scared or when we feel that we are in danger. Therefore, milk producing hormone is the gene that are turned once or twice in a lifetime.
Answer:
Describe two desert animals and the adaptation that help them survive?
Camel and Donkey
Explanation:
Both animals have two different types of stomach that enables them to store water for a very long time and makes them withstand drought
