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tresset_1 [31]
3 years ago
13

During the early stages of an enzyme purification protocol, when cells have been lysed but cytosolic components have not been se

parated, the reaction velocity versus substrate concentration is sigmoidal. As you continue to purify the enzyme, the curve shifts to the right. Explain your results.
Biology
2 answers:
Ksivusya [100]3 years ago
8 0

Answer:

This is an allosteric enzyme showing a double-displacement mechanism

(ping-pong mechanism) and during purification a heterotrophic activator is purified away.

Explanation:

Double-displacement mechanism involves the reaction in which no ternary complex is formed. An enzyme-substrate complex forms, a product leaves the complex, altered enzyme forms a second complex with another substrate molecule, and the second product leaves, regenerating the enzyme.

So, during the early stages of an enzyme purification protocol, when cells have been lysed but cytosolic components have not been separated, the reaction velocity versus substrate concentration is sigmoidal. As you continue to purify the enzyme, the curve shifts to the right, because of an allosteric enzyme showing a double-displacement mechanism and during purification, a heterotrophic activator is purified.

vesna_86 [32]3 years ago
4 0

Answer:

The enzyme is an allosteric enzyme that has double-displacement, thereby being purified a heterotrophic activator

Explanation:

The term heterotrophic activator refers to when the substrate and the regulator in a chemical reaction correspond to different molecules

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Answer:

Mendel's law of independent assortment

Explanation:

Gregor Mendel is a really important figure in genetics, his work on pea plants provided us with many of the fundamentals of genetics that we still have today!

Mendel proposed 3 laws:

1. The law of dominance - this law states that where there are two different alleles (heterozygous) the organism will always express the dominant trait over the recessive trait

2. The law of segregation - this law states that offspring will inherit one allele from each parent, because allele pairs separate in the process of meiosis, such that each gamete contains 1 allele of each trait. When the zygote is formed, it contains an allele from each parent.

3. The law of independent assortment - this law states that traits are independent from one another at the time of gamete formation. The genes are segregated separately from one another, as the presence of one does not impact the presence of another.

This example shows that all combinations of the height and color allele are possible, and therefore nicely demonstrates the law of independent assortment

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