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anastassius [24]
3 years ago
5

G you extracted your dna from cheek cells. the concentration of your dna sample is 250 ng/μl. the dna template for pcr should be

10 ng/μl. you should mix _____ μl of your dna sample and _____ μl of water to make final volume of 100 μl of dna that has the concentration of 10 ng/μl.
Biology
1 answer:
Karo-lina-s [1.5K]3 years ago
6 0
You should mix 4µl of DNA sample and complete it with 96µl (100-4) of water.

You can figure this out by using  C1.V1 = C2.V2
C1 = 250 <span>ng/μl (Sample Concentration)
</span>V1 = ?          (the necessary volume to take from the DNA sample)
C2 = 10 <span>ng/μl (PCR concentration)
</span>V2 = 100 µl (total volume)

V1 = \frac{C2 . V2}{C1} = \frac{10 . 100}{250} = 4µl

For the water: 100µl (total volume) = 4µl + Water
Water volume = 96µl
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