Answer:
The answer is D. Double Helix
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Out of the choices given, the mechanisms that drives evolution is natural selection. The correct answer is B, natural selection.
Answer:
I dont understand what you're asking but a Organisms niche is its instinctual behavior or what its learned in order to survive in its environment.
Explanation:
Answer:
In relation to voltage-gated ion channels, when depolarization occurs, Na⁺ gates open while K⁺ gates remain closed.
Explanation:
Depolarization is a phenomenon that occurs in cells like neurons, and depends on the distribution of ions in extracellular spaces and intraceluar.
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Na⁺ is a predominantly extracellular cation.
- K⁺ has a high intracellular concentration.
This distribution occurs when the membrane potential is at rest, with a negative intracellular value.
Depolarization involves opening the Na⁺ channels, so that this cation enters the intracellular space, causing a change in cell voltage. Increasing Na⁺ concentration in intracellular space progressively produces an action potential, resulting in an electrical signal.
While depolarization occurs, K⁺ channels remain closed, limiting the output of this cation to extracellular space, which would prevent the action potential from occurring.
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