While the absorbance at 420 nm is related to the amount of o-nitrophenol produced, the absorbance at 600 nm is proportional to cell density, which aids in standardizing our estimations of enzyme activity.
<h3>Describe absorbance.</h3>
The amount of light absorbed by a solution is measured by its absorbance (A), often referred to as optical density (OD). The amount of light that may flow through a solution is called its transmittance.
<h3>How is the activity of beta-galactosidase determined?</h3>
The colorless ONPG substrate is changed by beta-Galactosidase into galactose and the chromophore o-nitrophenol, which results in a vivid yellow solution. The amount of substrate transformed at 420 nm can be calculated by measuring the solution's beta-galactosidase activity using a spectrophotometer or a microplate reader.
<h3>What is measured by the beta-galactosidase assay?</h3>
The -Gal Assay Kit gives users the tools they need to swiftly assess the amounts of active beta-galactosidase expressed in cells that have been transfected with plasmids encoding the lacZ gene.
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Answer:
Google is great sometimes
Explanation:
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Answer:
The regular cells of the body tend to divide through the process of mitosis. However, the sex cells of the body divide through the process of meiosis.
In the process of mitosis, two daughter cells are formed which are identical to the parent cell and are diploid.
In the process of meiosis, four daughter cells are formed which are haploid and carry half the number of chromosomes as the parent cell.
A researcher is studying the science of attractiveness and asks volunteer test subjects to rank what trait they find most attractive in a person. What type of variable is the dependent variable in this experiment?
-Categoric
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- is a protein in metabolic reaction
- lowers activation energy
- is not changed during reaction
