It's know as chemical equilibrium
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Blood, flowers, bacteria, and skin
First episode release date was <span>February 27, 2012</span>
Answer: D. Replacing thermal power plants.
Explanation:
Thermal Power Stations are some of the biggest users of water in the world because water is integral to producing electricity in them. These stations burn fossil fuels like coal which then vaporizes water so that it becomes steam which then drives turbines that produce electricity.
Water is also used for cooling high temperatures produced.
If these thermal stations are replaced with something that uses less water, it will go a long way in keeping the water debt less than the water supply.
