Answer:
upper one is translocation and lower one is deletion
Explanation:
The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).
<span>For the purposes of this discussion, it is important to note that
elements are the basic components of Chemistry. Elements contain atoms.
All substances are made up of elements. Compounds are elements that
contain more than one type of atom that bond together via a chemical
reaction. Mixtures are made up of more than one substance, element or
compound that are not chemically bonded together. Compounds and mixtures
impact our daily lives because everything we have is made up of
elements. An example of a mixture is strawberry ice cream. Milk, cream,
and other elements are mixed together to make the ice cream. An example
of a compound is water. A droplet of water is made from two hydrogen
molecules (elements) and one oxygen molecule. </span>
B is the answer to this question!